Asprosin is a fasting-induced glucogenic protein that responds to low dietary glucose by stimulating hepatic glucose release. It is
secreted by white adipose tissue and circulates in the plasma at nanomolar levels. Asprosin- induced hepatic glucose production is
mediated by G protein-cAMP-protein kinase A pathway and is shown to be pathologically related with human and mouse insulin
Expressed in with total 176 AA. Mw: 20.0 KDa (calculated).
N-terminal 6xHis-tag and EK cleavage site, 36 extra AA (highlighted).
Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 8.0.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolves completely.
Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
ELISA and Western Blotting.