您当前所在位置: 首页 > 产品中心 > 重组蛋白

重组蛋白

Interleukin-13 (IL-13)

op

★Download Datasheet★

Origin:Recombinant

Cat. No.:42450

Tag: N-terminal 6xHis

Size: 0.1 mg

Source: E.coli

Purity: >95%

Other names: IL-13

Species: Mouse


Introduction

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth
muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages,
fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the up‑
regulation of CD23/Fc epsilon RII.

Description

Expressed in E.coli with total 135 AA. Mw: 15.2 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAPVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSL
TNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPF

Formulation

Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 7.0.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolves completely.

Storage

Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Application

ELISA and Western Blotting.

Endotoxin Level

<0.2 EU per 1 μg of the protein by the LAL method.