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Introduction
Fatty acid binding protein 4(FABP4), also termed adipocyte fatty acid binding protein (A-FABP), or aP2, is a novel
adipocyte-expressed factor which accounted for ~6% of total cellular proteins. Several animal experiments suggested that FABP-4
plays a key role in the link between obesity and various features of metabolic syndrome. Mice with targeted disruption of FABP-4
accompany FABP-5 almost completely protect against diet-induced obesity, insulin resistance, dyslipidemia, type 2 diabetes, and
fatty liver disease. Studies in human found FABP-4 serum levels were significantly increased in overweight and obese subjects,
which predicted the risk to develop metabolic syndrome and type 2 diabetes. Additionally, serum FABP-4 levels were associated
with carotid atherosclerosis and coronary artery disease.
Description
Total 160 AA. Mw: 18 KDa (calculated).
N-terminal His-tag and TEV cleavage site, 28 extra AA (highlighted).
Amino Acid Sequence
MSYYHHHHHHDYDIPTTENLYFQGAMGSMCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAG
MAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKS
TTIKRKREDDKLVVECVMKGVTSTRVYERA
Formulation
Lyophilized in 1 mg/mL in PBS.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Storage
Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Application
ELISA and Western blotting.
Endotoxin Level
<0.2 EU/μg.