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重组蛋白

Leptin protein (Tagless)

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Type: Recombinant

Cat. No.: 41B029

Tag: Tagless

Size: 0.1 mg

Source: E.Coli

Purity: >99%

Bioactive: Yes

Species: Human


Introduction

As one of the most important adipose derived hormones, Human Leptin is secreted primarily in the adipocytes of white adipose
tissue, and the level of circulating leptin is directly proportional to the total amount of fat in the body. It acts on receptors in the
hypothalamus of the brain where it inhibits appetite and has been shown to reduce body weight, food consumption, and plasma
glucose levels in various in vivo models.

Description

Recombinant human Leptin without Signal peptide (AA22-167), total 147 amino acids (AA). N-terminal His-tag removed. Mw:
16.1 kDa (calculated). 1 extra AA left (In bold).

Amino Acid Sequence

AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQIS
NDLENLRDLLHVLAFSKSCHLPWASG LETLDSLGGVLEASGYSTEVVALSRLQGSLQDM LWQLDLSPGC

Formulation

Lyophilized in 1 mg/mL in PBS.

Reconstitution

Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve
completely.


Storage

Store at –20°C. Avoid repeated freezing/thawing cycles.

Endotoxin Level

<0.2 EU/ug.

Application

Cell culture, animal studies, ELISA and Western blotting.