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重组蛋白

Leptin protein (Tagless)

op

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Type: Recombinant

Cat. No.: 41B039

Tag: Tagless

Size: 0.1 mg

Source: E.Coli

Purity: >99%

Bioactive: Yes

Species: Mouse


Introduction

Encoded by the ob (obese) gene, Leptin is an adipose-derived cytokine that suppresses appetite and increases thermogenesis.
Mouse Leptin shares approximately 84% sequence identity with the human protein. Mice with mutations in the obese gene that block
the synthesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature.

Description

Recombinant Mouse Leptin without Signal peptide (AA22-167), total 147 amino acids (AA). N-terminal His-tag removed.
Mw: 16.1 kDa
(calculated). 1 extra AA left (In bold).

Amino Acid Sequence

AVPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAF
SKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDI LQQLDVSPEC

Formulation

Lyophilized in 1 mg/mL in PBS.

Reconstitution

Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve
completely.


Storage

Store at –20°C. Avoid repeated freezing/thawing cycles.

Endotoxin Level

<0.2 EU/ug.

Application

Cell culture, animal studies, ELISA and Western blotting.