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| Species: Mouse |
Introduction
PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids.
These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe
through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may
indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.
Description
Expressed in HEK293 cells with total 516 AA. Mw: 57.34 KDa (calculated). C-terminal 6xHis-tag and TEV cleavage site, 13 extra AA (highlighted).
Recombinant antigen for research use or manufacturing only.
Amino Acid Sequence
MAELLASLPAWAAVLLLFFATVSGSTGPRSRENRGASRIPSQFSEEERVAIKEALKGAIQIPTVSFSHEESNTTALA
EFGEYIRKAFPTVFHSSLVQHEVVAKYSHLFTIQGSDPSLQPYMLMAHIDVVPAPEEGWEVPPFSGLERNGFIYG
RGALDNKNSVMAILHALELLLIRNYSPKRSFFIALGHDEEVSGEKGAQKISALLQARGVQLAFLVDEGSFILEGFIP
NLEKPVAMISVTEKGALDLMLQVNMTPGHSSAPPKETSIGILSAAVSRLEQTPMPNMFGGGPLKKTMKLLANEF
SFPINIVLRNLWLFHPIVSRIMERNPITNALVRTTTALTMFNAGIKVNVIPPLAQATINCRIHPSQTVHEVLELVKNTVA
DDRVQLHVLRSFEPLPISPSDDQAMGYQLLQETIRSVFPEVDIVVPGICIANTDTRHYANITNGMYRFNPLPLNPQD
FSGVHGINEKVSVQNYQNQVKFIFEFIQNADTYKEPVPHLHELENLYFQGHHHHHH
Formulation
Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 8.0.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Storage
Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Application
ELISA and Western Blotting.
Endotoxin Level
<0.2 EU per 1 μg of the protein by the LAL method.