In an UCP1-independent manner, maybe
through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may
indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.
Lyophilized at 1 mg/mL in NaCl 500mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 8.0.
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Store lyophilized protein at -20°C. Aliquot reconstituted protein and store at -80°C. Avoid repeated freezing/thawing cycles.
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
ELISA and Western Blotting.
<0.2 EU per 1 μg of the protein by the LAL method.