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重组蛋白

Procalcitonin (PCT)

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Origin: Recombinant

Cat. No.: 41670

Tag: N-terminal 6xHis

Size: 0.1 mg

Source: E.coli

Purity: >90%

Other names: PCT

Species: Human


Introduction
Procalcitonin is a prohormone of calcitonin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or
shock. Procalcitonin is also associated with systemic complications in humans suffering from acute pancreatitis.

Description

Expressed in E.coli with total 152 AA. Mw: 16.9 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 36 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Amino Acid Sequence

MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKAS
ELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN

Formulation

Lyophilized at 1 mg/mL in PBS.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolves completely.

Storage

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Application

Standard ELISA test, Western Blot.

Endotoxin Level

<0.2 EU/μg