Monoclonal Antibody against Human LCN2 (Clone 2G1)
Size: 0.1 mg
Introduction
Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), 24p3, or neutrophil lipocalin (NL), is a 25-kDa secretory glycoprotein [1]. LCN2 has been implicated in a variety of cellular processes including the innate immune response, differentiation, tumorigenesis, and cell survival [2-3]. It appears to be upregulated in various inflammation and infection conditions. Several reports suggest that LCN2 may represent a sensitive biomarker for various renal injuries [4] and is associated with several types of cancers, including breast cancer [5], ovarian, colorectal, and pancreatic cancers [6-7]. Furthermore, a growing body of evidence suggests that serum levels of LCN2 are correlated with obesity, insulin resistance, hyperglycemia, coronary heart disease and fatty liver disease in humans [8-11].
Purification
Protein G affinity purification
Immunogen
Recombinant full-length human lipocalin-2 (Cat. No: 41050) in E.coli.
Specificity
The antibody detects human LCN2.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Application/Usage
This antibody can be used as a capture antibody in a human lipocalin-2 ELISA in combination with monoclonal anti-human lipocalin-2 antibody clone 2C3 (Cat. No.: 21051) as detection antibody.
References
[1] Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10425-32
[2] Yang, J. et al. (2002) Mol. Cell. 10:1045-56.
[3] Flo, T.H. et al. (2004) Nature 432:917-21.
[4] Mishra, J. et al. (2005) Lancet 365:1231-6.
[5] Yang, J. et al. (2009) Proc Natl Acad Sci U S A 106(10):3913-8.
[6] Hanai, J. et al. (2005) J. Biol. Chem. 280:13641-7.
[7] Lee, H.J. et al. (2005) Int. J. Cancer 118:2490-7.
[8] Wang Y, et al. (2007) Clin Chem 53(1):34-41.
[9] Yan QW, et al. (2007) Diabetes 56(10):2533-40.
[10] Ruby LC Hoo, et al. (2008) Expert Rev. Endocrinol Metal 3(1):29-41.
[11] Milner KL, et al. Hepatology [Epub ahead of print].