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混合性结缔组织病 (Mixed Connective Tissue Disease, MCTD)

U1snRNP A

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Cat. No.: 41510

Other Names:   U1-snRNPA, RNPA, RNP-A

Species: Human

Size: 0.1mg

Origin: Recombinant

Tag: N-terminal 6xHis

Source: Spodoptera frugiperda Sf9

Purity: >90%


Introduction

Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules.
U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins, and the three 68/70 kDa (snRNP68/70), A polypeptides (snRNPA) and C polypeptides (snRNPC) are unique to the U1-snRNP particle. 
Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with SLE.


Immunological Function
As an autoantigen, RNP-A binds with IgG-type human auto-antibodies.

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQ
AFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGA
TPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLS
ENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKIS
FAKK



Applications
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Formulation
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

Description

Expressed in insect Sf9 cells with a total of 306 AA.

Mw: 34.2 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Storage
Store at –80°C. Avoid repeated freezing /thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.