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自身免疫性糖尿病 (Autoimmune Diabetes)

GAD65 (1-95aa truncated)

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Cat. No.: 41847

Abbreviation: GAD65-(1-95tr)

Species: Human

Size: 0.1mg

Origin: Recombinant

Tag: N-terminal 6xHis

Source: Spodoptera frugiperda Sf9

Purity: >95%


Introduction

GAD65 is primarily expressed in neuron cells and pancreas β-cells. It works as a catalyser in GABA synthesis. Glutamic acid
decarboxylase autoantibodies (GADA) are found in 70% to 80% of individuals with new-onset type 1 diabetes, making it the most
frequent autoantibody in autoimmune diabetes. GADA can be detected in serum for many years post diagnosis, and high
concentrations of GADA have been considered as a marker of faster β-cell exhaustion in these patients. Furthermore, GADA in
non-diabetic individuals predicts the later development of type 1 diabetes1. Besides autoimmune diabetes, GADA also exists in Stiff Man Syndrome, autoimmune poly-endocrinopathies, and some of Grave’s Disease patients.


Immunological Function
As an autoantigen, GAD65 binds with IgG-type human auto-antibodies.

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGADLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILMHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMMIARFKMFPEVKEKGMAALPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEAKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQNCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHVDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWYIPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL

Applications
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Formulation
Liquid in Phosphate buffer containing NaCl (1M), Na2HPO4 (10mM), KCl (2.7mM) and KH2PO4 (2.0mM) with protease inhibitor,
pH8.0.


Description

Expressed in Baculovirus-sf9 vector expression system with a total of 515 amino acids (AA).

Mw: 58.8 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Storage
Store at –80°C. Avoid repeated freezing /thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.