您当前所在位置: 首页 > 产品中心 > 高质量自免抗原

自身免疫性肝炎&原发性胆汁性胆管 (AIH & PBC)

PDC-E2

op

★Download Datasheet★

Cat. No.: 41A082

Other Names:   PDC-E2

Species: Human

Size: 0.1mg

Origin: Recombinant

Tag: N-terminal 6xHis

Source: Spodoptera frugiperda Sf9

Purity: >90%


Introduction

The pyruvate dehydrogenase complex (PDC) is a mitochondrial multienzyme complex involved in the maintenance of the cellular
redox state. PDC-E2 is the central E2 component of this complex. The anti-PDC-E2 antibodies belong to the group of
Anti-Mitochondrial Antibodies (AMA-M2) and are strongly associated with PBC.


Immunological Function

As an autoantigen, PDC-E2 binds with IgG-type human auto-antibodies.

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGASLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLEKPITMLL

Applications
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Formulation
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

Description

Expressed in insect Sf9 cells with a total of 586 AA.

Mw: 62.72 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Storage
Store at –80°C. Avoid repeated freezing /thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.