Polyclonal Antibody against Mouse Adiponectin
Size: 0.1 mg
Introduction
Adiponectin, also termed gelatin-binding protein-28 (GBP28), AdipoQ, ACP30 (Acrp30), or apM, is a major adipocyte-secreted
adipokine which is abundantly present in the circulation as three distinct oligomeric complexes: LMW (67kDa), MMW (167kDa) and
HMW (300kDa) adiponectin. Its levels are decreased in insulin resistance, diabetes and cardiovascular disease. Conversely,
elevation of circulating adiponectin concentrations can alleviate various vascular dysfunctions in animal models, suggesting this
adipokine exerts vasculoprotective effects. In addition, adiponectin may also be of importance in the development and progression of several malignancies.
Purification
Antigen affinity-purified
Immunogen
Recombinant full-length mouse adiponectin expressed in mammalian cells.
Specificity
The antibody detects mouse/rat adiponectin.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at -20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Application/Usage
Western blot - This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse/rat adiponectin.
ELISA - This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse/rat adiponectin.
References
[1] Xu A, et al. (2005) Testosterone selectively reduces the high molecular weight form of adiponectin by inhibiting its secretion from adipocytes. J. Biol. Chem. 280, 18073–18080
[2] Xu A, et al. (2008) Selective Elevation of Adiponectin Production by the Natural Compounds Derived from a Medicinal Herb Alleviates Insulin Resistance and Glucose Intolerance in Obese Mice. Endocrinology. [Epub ahead of print]
[3] Xu A, et al. (2004) Adiponectin ameliorates dyslipidemia induced by the human immunodeficiency virus protease inhibitor ritonavir in mice. Endocrinology. 145(2):487-94
[4] Wang Y, et al. (2008) Post-translational modifications of adiponectin: mechanisms and functional implications. Biochem J. 409(3):623-33
Publications Citing This Product
1. Hui X, Gu P, Zhang J, Nie T, Pan Y, Wu D, Feng T, Zhong C, Wang Y, Lam KS, Xu A. Adiponectin enhances cold-induced browning of subcutaneous adipose tissue via promoting M2 macrophage proliferation. Cell metabolism. 2015 Aug 4;22(2):279-90.
2. Ng RC, Cheng OY, Jian M, Kwan JS, Ho PW, Cheng KK, Yeung PK, Zhou LL, Hoo RL, Chung SK, Xu A. Chronic adiponectin deficiency leads to Alzheimer’s disease-like cognitive impairments and pathologies through AMPK inactivation and cerebral insulin resistance in aged mice. Molecular neurodegeneration. 2016 Dec;11(1):1-6.
3. Zhang X, Cheng Q, Wang Y, Leung PS, Mak KK. Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin. Cell Death & Differentiation. 2017 Feb;24(2):225-37.
4. Geng L, Liao B, Jin L, Huang Z, Triggle CR, Ding H, Zhang J, Huang Y, Lin Z, Xu A. Exercise alleviates obesity-induced metabolic dysfunction via enhancing FGF21 sensitivity in adipose tissues. Cell reports. 2019 Mar 5;26(10):2738-52.