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Polyclonal Antibody against Human Adiponectin
Size: 0.1 mg
Introduction
Adiponectin, also termed gelatin-binding protein-28 (GBP28), AdipoQ, ACP30 (Acrp30), or apM, is a major adipocyte-secreted adipokine which is abundantly present in the circulation as three distinct oligomeric complexes: LMW (67kDa), MMW (167kDa) and HMW (300kDa) adiponectin. Its levels are decreased in insulin resistance, diabetes and cardiovascular disease. Conversely, elevation of circulating adiponectin concentrations can alleviate various vascular dysfunctions in animal models, suggesting this adipokine exerts vasculoprotective effects. In addition, adiponectin may also be of importance in the development and progression of several malignancies.
Purification
Rabbit crude IgG was purified by protein-G chromatography.
Immunogen
Recombinant full-length human adiponectin expressed in mammalian cells.
Specificity
The antibody detects three types of circular human adiponectin and monomer (30kDa) adiponectin.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at -20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Application/Usage
Western blot - This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human adiponectin.
ELISA - This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human adiponectin.