Polyclonal Antibody against Human FGF-21
Size: 0.1 mg
Introduction
Fibroblast growth factor 21 (FGF-21) is a novel protein that has been implicated in the regulation of lipid and glucose metabolism
under fasting and ketotic conditions [1,2]. In murine models, FGF-21 is predominantly expressed in liver, but also expressed in
adipose tissue and pancreatic β-cells [3,4]. FGF-21 stimulates glucose uptake in adipocytes. It also protects animals from
diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to
diabetic rodents [5]. When administered daily for 6 weeks to diabetic rhesus monkeys, FGF-21 caused a dramatic decline in fasting
plasma glucose, fructosamine, triglycerides, insulin and glucagon [6]. Furthermore, elevated plasma FGF-21 concentrations in
humans appear to be related to the presence of hepatic and peripheral insulin resistance [7].
Purification
Antigen affinity-purified
Immunogen
Recombinant full-length human FGF-21 expressed in E.coli.
Specificity
The antibody detects circular human FGF-21.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Application/Usage
ELISA - The antibody can be used at 2 μg /mL with the appropriate secondary reagents to detect human FGF21.
References
[1] Kharitonenkov A, Shiyanova TL, et al. (2005) J Clin Invest; 115: 1627– 1635
[2] Badman MK, Pissios P, et al. (2007) Cell Metab; 5: 426– 437
[3] Nishimura T, Nakatake Y, et al. (2000) Biochim Biophys Acta; 1492: 203– 206
[4] Kurosu H, Choi M, et al. (2007) J Biol Chem; 282: 26687– 26695
[5] Kharitonenkov A, Shiyanova TL, et al. (2005) J. Clin. Invest. 115: 1627–35.
[6] Kharitonenkov A, Wroblewski VJ, et al. (2007) Endocrinology;148:774-81
[7] Chavez AO, Molina-Carrion M, et al. (2009) Diabetes Care; 32:1542-6.
Publications Citing This Product
1. Ye D, Li H, Wang Y, Jia W, Zhou J, Fan J, Man K, Lo C, Wong C, Wang Y, Lam KS. Circulating fibroblast growth factor 21 is a sensitive biomarker for severe ischemia/reperfusion injury in patients with liver transplantation. Scientific reports. 2016 Jan 25;6(1):1-9.