Polyclonal Antibody against Mouse IL-33
Size: 0.1 mg
Introduction
IL‑33 was identified based on sequence and structural homology with IL‑1 family cytokines. The N‑terminal portion of full-length
IL‑33 contains a predicted bipartite nuclear localization sequence and a homeodomain‑like helix‑turn‑helix DNA binding domain.The C‑terminal fragment, corresponding to mature IL‑33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtimeorphan
receptor involved in the augmentation of Th2 cell responses. A ternary signaling complex is formed by the subsequent association of
IL‑33 and ST2L with IL‑1R AcP. Stimulation of Th2 polarized lymphocytes with mature IL‑33 in vitro induces IL‑5 and IL‑13 secretion. In vivo administration of mature IL‑33 promotes increased production of IL‑5, IL‑13, IgE, and IgA, as well as splenomegaly and
inflammatory infiltration of mucosal tissues.
Purification
Antigen affinity-purified
Immunogen
Recombinant mouse IL-33 (Cat. No.: 41750) in E.coli.
Specificity
The antibody detects mouse IL-33 in ELISA.
Formulation
Liquid in phosphate-buffered saline (PBS).
Storage
Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.
Application/Usage
ELISA - This antibody can be used as capture antibody in Mouse IL-33 ELISA (Cat. No.: 31750).