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自身免疫性肝炎&原发性胆汁性胆管 (AIH & PBC)

LC1

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Cat. No.: 41A052

Other Names:   LC1, FTCD

Species: Human

Size: 0.1mg

Origin: Recombinant

Tag: N-terminal 6xHis

Source: Spodoptera frugiperda Sf9

Purity: >90%


Introduction
LC1 is an enzyme which catalyzes the conversion of formiminoglutamate and tetrahydrofolate into formiminotetrahydrofolate and
glutamate. As a bio-functional enzyme of tetrahydrofolate synthesis, it is the target antigen of anti-LC1 (liver cytosol antigen type 1)
autoantibodies. Presence of LC1 autoantibodies is a marker for type 2 autoimmune hepatitis.

Immunological Function
As an autoantigen,  LC1 binds with IgG-type human auto-antibodies.

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGASQLVECVPNFSEGKNQEVIDAISGAITQTPGCVLLDVDAGPSTNRTVYTFVGPPECVVEGALNAARVASRLIDMSRHQGEHPRMGALDVCPFIPVRGVSVDECVLCAQAFGQRLAEELDVPVYLYGEAARMDSRRTLPAIRAGEYEALPKKLQQADWAPDFGPSSFVPSWGATATGARKFLIAFNINLLGTKEQAHRIALNLREQGRGKDQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLDAAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGARSAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYLEAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE

Applications
Standard ELISA test, line/dot assay and microarray assay with positive/negative sera panels.

Formulation
Liquid in storage buffer (50mM Tris, 300-500mM NaCl, 10% Glycerol, Protease inhibitor, pH8.0).

Description

Expressed in insect Sf9 cells with a total of 565 AA.

Mw: 61.9 KDa (calculated).

N-terminal 6xHis-tag and TEV cleavage site, 25 extra AA (highlighted).

Recombinant antigen for research use or manufacturing only.

Storage
Store at –80°C. Avoid repeated freezing /thawing cycles.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.